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. Author manuscript; available in PMC: 2017 Apr 14.
Published in final edited form as: J Neurosci Methods. 2017 Feb 13;280:64–76. doi: 10.1016/j.jneumeth.2017.02.002

Figure 4. Solubilization with optimized lysis buffer preserves phospho-specific signals for incubations up to 18 hrs.

Figure 4

(A) Total ERK1/2 protein does not change despite extended incubations, consistent with no changes in total protein during these same incubations (Fig. 3C). (B) Phospho-specific ERK1/2 signal is significantly enhanced by incubations at lower temperatures for up to 18 hrs. Representative Western blots of the data quantified are depicted in A′–B′. In B′, total ERK1/2 appears red and phospho-ERK1/2 (pERK) appears yellow (green overlaid on red) and is most strongly recognized in the 42kDa band (arrow).