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. Author manuscript; available in PMC: 2017 Apr 14.
Published in final edited form as: J Neurosci Methods. 2017 Feb 13;280:64–76. doi: 10.1016/j.jneumeth.2017.02.002

Figure 6. TRIzol-precipitated protein from brain tissue represents multiple cell types and protein classes.

Figure 6

(A) Different cell types inherent in brain tissue are detected in Western blot analysis of precipitated protein. NeuN=neurons, GFAP=astrocytes, Olig2=oligodendrocytes, Iba1=microglia, PV=inhibitory interneurons. (B) Different protein classes are represented in precipitated protein, including synaptic (PSD95, VGlut1), membrane bound (Iba1), nuclear (NeuN), cytoplasmic (actin and ERK1/2 in Fig. 4), and secreted (IL-10) proteins. (C) Protein from specific populations of a single cell suspension (i.e. microglia in the CD11b+ population and other neural cell types in the CD11b− population) is successfully analyzed after solubilization in optimized lysis buffer. The CD11b+ population contains Iba1, but not NeuN protein, while the CD11b− population contains NeuN, but not Iba1 protein.