Table 1.
Primary antibody details for Western blotting analysis
| Dilution | Company | Catalog # | Host | Clonality | |
|---|---|---|---|---|---|
| ERK1/2 | 1:5000 | Cell Signaling | 4696S | Mouse | Monoclonal |
| Phospho-ERK1/2 | 1:2000 | Cell Signaling | #4370S | Rabbit | Monoclonal |
| GFAP | 1:2000 | Millipore | MAB5628 | Mouse | Monoclonal |
| Iba1 | 1:2000a 1:500b |
Wako | 019-19741 | Rabbit | Polyclonal |
| NeuN | 1:2000a 1:500b |
Bioss | bs-1613R | Rabbit | Polyconal |
| PSD95 | 1:2000 | ThermoFischer Scientific | 51-6900 | Rabbit | Polyclonal |
| β-actin | 1:10,000 | Abcam | ab8227 | Rabbit | Polyclonal |
| IL-10 | 1:100 | Acris | AP52181PU-N | Rabbit | Polyclonal |
| Parvalbumin | 1:2000 | Abcam | ab11427 | Rabbit | Polyclonal |
| Olig2 | 1:500 | Abcam | ab109186 | Rabbit | Monoclonal |
| VGlut1 | 1:1000 | Millipore | Ab5905 | Guinea Pig | Polyclonal |
a
Concentration for tissue homogenate;
b
Concentration for dissociated cell sample