Table 4.
Comparison of chemical composition of buffers commonly used for protein analyses
| Optimized | RIPA | 1% SDS | Standard | |
|---|---|---|---|---|
| EDTA (mM) | 20 | 1 | ||
| NaCl (mM) | 140 | 150 | 140 | |
| SDS (%) | 5 | 0.1 | 1 | 2 |
| Tris (mM) | 100 | 50 | 10 | |
| Triton-X100 (%) | 1 | |||
| Sodium Deoxycholate (%) | 0.5 |
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