Table 3. Genes investigated by Human Th1-Th2-Th3 RT2 Profiler™ PCR Array, and changes in their expression induced by atorvastatin.
| Position | Symbol | Description | Fold Regulation |
|---|---|---|---|
| A01 | IL17A | Interleukin 17A | −1,0822 |
| A02 | CCL11 | Chemokine (C-C motif) ligand 11 | 1,0622 |
| A03 | CCL5 | Chemokine (C-C motif) ligand 5 | −1,1696 |
| A04 | CCL7 | Chemokine (C-C motif) ligand 7 | 1,0822 |
| A05 | CCR2 | Chemokine (C-C motif) receptor 2 | -3,4822 |
| A06 | CCR3 | Chemokine (C-C motif) receptor 3 | 2,5974 |
| A07 | CCR4 | Chemokine (C-C motif) receptor 4 | 1,0310 |
| A08 | CCR5 | Chemokine (C-C motif) receptor 5 | −1,6656 |
| A09 | CD28 | CD28 molecule | −1,1859 |
| A10 | CD4 | CD4 molecule | −2,0648 |
| A11 | CD40LG | CD40 ligand | −2,6500 |
| A12 | IL23A | Interleukin 23, alpha subunit p19 | −1,4804 |
| B01 | CD80 | CD80 molecule | 1,0098 |
| B02 | CD86 | CD86 molecule | -7,4436 |
| B03 | CEBPB | CCAAT/enhancer binding protein (C/EBP), beta | −1,2025 |
| B04 | CREBBP | CREB binding protein | 1,0973 |
| B05 | CSF2 | Colony stimulating factor 2 (granulocyte-macrophage) | 1,0822 |
| B06 | CTLA4 | Cytotoxic T-lymphocyte-associated protein 4 | 1,0381 |
| B07 | CXCR3 | Chemokine (C-X-C motif) receptor 3 | −1,1065 |
| B08 | FASLG | Fas ligand (TNF superfamily, member 6) | −1,5648 |
| B09 | GATA3 | GATA binding protein 3 | −1,2710 |
| B10 | GFI1 | Growth factor independent 1 transcription repressor | −1,3250 |
| B11 | GLMN | Glomulin, FKBP associated protein | −1,5433 |
| B12 | GPR44 | G protein-coupled receptor 44 | −1,9807 |
| C01 | HAVCR2 | Hepatitis A virus cellular receptor 2 | −1,7484 |
| C02 | ICOS | Inducible T-cell co-stimulator | −1,2277 |
| C03 | IFNG | Interferon, gamma | −1,7487 |
| C04 | IGSF6 | Immunoglobulin superfamily, member 6 | −2,2439 |
| C05 | IL10 | Interleukin 10 | 1,9411 |
| C06 | IL12B | Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) | −1,1859 |
| C07 | IL12RB2 | Interleukin 12 receptor, beta 2 | −1,1519 |
| C08 | IL13 | Interleukin 13 | −1,0396 |
| C09 | IL13RA1 | Interleukin 13 receptor, alpha 1 | -3,6200 |
| C10 | IL15 | Interleukin 15 | −1,6313 |
| C11 | IL18 | Interleukin 18 (interferon-gamma-inducing factor) | -5,8401 |
| C12 | IL18R1 | Interleukin 18 receptor 1 | −1,1696 |
| D01 | IL1R1 | Interleukin 1 receptor, type I | −1,1455 |
| D02 | IL1R2 | Interleukin 1 receptor, type II | 1,2693 |
| D03 | IL2 | Interleukin 2 | −2,3554 |
| D04 | IL2RA | Interleukin 2 receptor, alpha | −1,4702 |
| D05 | IL4 | Interleukin 4 | 1,0822 |
| D06 | IL4R | Interleukin 4 receptor | −1,2108 |
| D07 | IL5 | Interleukin 5 (colony-stimulating factor, eosinophil) | −1,7243 |
| D08 | IL6 | Interleukin 6 (interferon, beta 2) | -5,9628 |
| D09 | IL6R | Interleukin 6 receptor | −1,2277 |
| D10 | IL7 | Interleukin 7 | −1,3159 |
| D11 | IL9 | Interleukin 9 | 1,0822 |
| D12 | INHA | Inhibin, alpha | 1,0822 |
| E01 | INHBA | Inhibin, beta A | 1,4682 |
| E02 | IRF1 | Interferon regulatory factor 1 | 1,1761 |
| E03 | IRF4 | Interferon regulatory factor 4 | −1,0324 |
| E04 | JAK1 | Janus kinase 1 | −1,0324 |
| E05 | JAK2 | Janus kinase 2 | −1,0614 |
| E06 | LAG3 | Lymphocyte-activation gene 3 | −1,4103 |
| E07 | LAT | Linker for activation of T cells | 1,0238 |
| E08 | MAF | V-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) | −1,0468 |
| E09 | MAP2K7 | Mitogen-activated protein kinase kinase 7 | −1,1942 |
| E10 | MAPK8 | Mitogen-activated protein kinase 8 | 1,0028 |
| E11 | NFATC1 | Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 | −1,3159 |
| E12 | NFATC2 | Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 | −1,0468 |
| F01 | NFATC2IP | Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein | −1,2799 |
| F02 | PCGF2 | Polycomb group ring finger 2 | −1,7005 |
| F03 | PTPRC | Protein tyrosine phosphatase, receptor type, C | −1,1696 |
| F04 | SFTPD | Surfactant protein D | 3,1037 |
| F05 | SOCS1 | Suppressor of cytokine signaling 1 | 1,0098 |
| F06 | SOCS2 | Suppressor of cytokine signaling 2 | −1,5977 |
| F07 | SOCS5 | Suppressor of cytokine signaling 5 | 1,0747 |
| F08 | SPP1 | Secreted phosphoprotein 1 | -5,2634 |
| F09 | STAT1 | Signal transducer and activator of transcription 1, 91kDa | 1,0673 |
| F10 | STAT4 | Signal transducer and activator of transcription 4 | −1,0973 |
| F11 | STAT6 | Signal transducer and activator of transcription 6, interleukin-4 induced | 1,0028 |
| F12 | TBX21 | T-box 21 | −1,0913 |
| G01 | TFCP2 | Transcription factor CP2 | −1,3813 |
| G02 | TGFB3 | Transforming growth factor, beta 3 | 1,1127 |
| G03 | TLR4 | Toll-like receptor 4 | -3,4822 |
| G04 | TLR6 | Toll-like receptor 6 | −1,4702 |
| G05 | TMED1 | Transmembrane emp24 protein transport domain containing 1 | −1,4499 |
| G06 | TNF | Tumor necrosis factor | 1,0973 |
| G07 | CD27 | CD27 molecule | −1,2025 |
| G08 | TNFRSF8 | Tumor necrosis factor receptor superfamily, member 8 | -5,4869 |
| G09 | TNFRSF9 | Tumor necrosis factor receptor superfamily, member 9 | −1,5757 |
| G10 | TNFSF4 | Tumor necrosis factor (ligand) superfamily, member 4 | 1,0098 |
| G11 | TYK2 | Tyrosine kinase 2 | −1,1535 |
| G12 | YY1 | YY1 transcription factor | −1,3909 |
For each condition (untreated/treated with atorvastatin) a pool of RNA was constituted, starting with the same amount of RNA (500 ng) from each patient of Cohort 1 (see Methods).
Data are presented as fold of changes in gene expression induced by atorvastatin treatment as compared with the gene expression in untreated CD4+T-cells. Increased genes are indicated in red and decreased genes in green. A fold change in gene expression of ≥ 3 was taken as significant.