Table 4. Oligonucleotide primers used for real time quantitative polymerase chain reaction.

GeneBank accession number	Gene name	Primers 5’-3’
NM_001964	Early growth response (EGR1)	For GAGCAGCCCTACGAGCAC Rev GTCTCCACCAGCACCTTCTC
NM_005252	V-fos FBJ murine osteosarcoma viral oncogene homolog (FOS)	For GACCTTATCTGTGCGTGAA Rev CACTGGGAACAATACACACT
NM_001123396	Chemokine (C-C motif) receptor 2 (CCR2)	For GCATTCAGCCAGGAGATG Rev ATCATCGGACTCCACCAA
NM_138554	Toll-like receptor 4 (TLR4)	For GCCCTGCGTGGAGGTGGTT Rev GGGGAGGTTGTCGGGGATTTTGT
NM_004048	β-2-Microglobulin (B2M)	For AGGACTGGTCTTTCTATCTCTTGT Rev ACCTCCATGATGCTGCTTACA
NM_002046	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	For AGTCAGCCGCATCTTCTT Rev GCCCAATACGACCAAATCC

Confirmation and validation of candidate genes among those reduced or increased by atorvastatin was performed by real time quantitative polymerase chain reaction (RT-qPCR) after reverse transcription of single-patient RNA.