Table 6.
The list of identified differentially expressed protein spots in rCHO cells supplemented with mix. # 8, 10, compared to control group by MALDI-TOF/TOF
| Spot no.a | Protein Name | Accession no.b | Theoretical Mr/pI | Protein score | Peptide count | Sequence coverage (%) | Fold change Mix 8/C | Fold change Mix 10/C |
|---|---|---|---|---|---|---|---|---|
| 2540 | Vimentin | gi|860908 | 44611/4.75 | 416 | 6 | 16 | 1.7 | 2 |
| 1629 | NDRG1 | gi|344259130 | 35603/5.89 | 106 | 1 | 4 | 1.5 | 1.8 |
| 2171 | pyruvate dehydrogenase E1 | gi|625199086 | 39732/6.41 | 280 | 5 | 18 | 1.4 | 1.5 |
| 2042 | 60S acidic ribosomal protein P0 isoform X2 | gi|344237054 | 30039/8.68 | 339 | 5 | 27 | 0.6 | - |
| 2441 | Proteasome subunit alpha type-3 | gi|344250391 | 29667/6.38 | 132 | 2 | 8 | 0.6 | - |
| 2762 | ferritin heavy chain | gi|625224189 | 21644/5.74 | 323 | 4 | 30 | 1.5 | 1.5 |
| 2524 | triosephosphate isomerase isoform X1 | gi|625183009 | 32313/5.36 | 487 | 5 | 23 | 0.7 | - |
| 2602 | thioredoxin-dependent peroxide reductase | gi|354476011 | 28336/6.79 | 174 | 2 | 17 | - | 1.4 |
| 1517 | Gluthation synthetase-like | gi|537151483 | 53458/5.4 | 372 | 4 | 13 | 0.6 | - |
| 2690 | Cytidine monophosphate (UMP-CMP) kinase 1 | gi|17389257 | 26125/8.13 | 134 | 2 | 14 | 1.4 | 1.3 |
a
Spot numbers belonged to the identified spots in 2DE gels indicated in Figure 2;
b
protein accession numbers were obtained from Cricetulus griseus using MASCOT peptide Mass Fingerprint peptide software.