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. 2017 Apr 13;5:e3174. doi: 10.7717/peerj.3174

Figure 2. Induction of Pluripotent cells from OSCC.

Figure 2

(A) Timeline of iPSCs induction. (B-i) Morphology of H103. (B-ii) Morphology of transduced H103 at 24 h post infection. (B-iii) Emergence of iPSCs like colonies from reprogrammed H103 on MEF feeder layer at day 15 (Red arrow). (B-iv-v) Images of established stable H103 iPSCs like cells at passage number 10. (C-i) Morphology of H376. (C-ii) Morphology of transduced H376 at 24 h post infection. (C-iii) Emergence of iPSC like colonies from H376 on MEF feeder layer at day 15 (Red arrow). (C-vi) Image of iPSC like cells from H376 at P2. (C-v) Differentiated reprogrammed H376 (Red arrows), collected at P5 and P10 for real time expressions analysis.