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. 2017 Apr 13;5:e3174. doi: 10.7717/peerj.3174

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©2017 Verusingam et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A-i) Control parental H103 cells showing negative for the Alizarin red staining (A-ii) H103 iPSCs like cells showing positive for the Alizarin red staining indicating calcium deposits (Red arrow). (A-iii) Negative staining for Oil-Red-O in control parental H103 cells. (A-iv) Adipogenesis induced lipid droplets observed red in colour stain after Oil-O-Red staining. (Red arrow) indicates the tiny lipid droplets. Nikon inverted microscope, original magnification: (A-i & ii) 10×, (A-iii & iv) 40×.