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. Author manuscript; available in PMC: 2018 Feb 22.
Published in final edited form as: J Am Chem Soc. 2017 Feb 13;139(7):2750–2756. doi: 10.1021/jacs.6b12284

Figure 2.

Figure 2

Trapping of DNA and analysis of base flipping at a C:C mismatch site within a DNA duplex. (A) The DNA duplex is driven into the αHL nanopore under an applied potential where it is held for up to 20 s and then ejected by reversing the applied bias. While resident within the pore, the C:C mismatch site is aligned with the latch constriction of αHL. (B) Modulating current signatures are observed while DNA resides within the nanopore, where I1 corresponds to a confirmation where all bases are intrahelical and I2 corresponds to a conformation where one of the cytosine bases at the mismatch site is extrahelical. Intra- and extrahelical lifetimes are given by t1 and t2, respectively. Uninterrupted current-time traces demonstrating sequential capture and release of multiple DNA duplexes are shown in Figures S1 and S2.