Figure 5. Mutations to the CTS reduces cilia targeting.

A) Representative diagram of mutation on 5-HT₆ receptor to create a receptor without the primary cilia targeting sequence (Δ5-HT₆ No CTS). B) Representative diagram of mutation on 5-HT₆ receptor to create a receptor with 5-HT₇ receptor intracellular loop 3 (IC3) (Δ5-HT₆ i3-7). C) Proportion of receptor localized in and out of primary cilia in 5-HT₆KO cultured neurons transfected with 25% receptor plasmid: WT 5-HT₆ (n=39 neurons), Δ5-HT₆ No CTS (n=15 neurons), or Δ5-HT₆ i3-7 (n=18 neurons). D) Average primary cilia lengths when the mutant 5-HT₆ receptors localize to cilia: WT 5-HT₆ (n=43 neurons), Δ5-HT₆ No CTS (n=28 neurons), or Δ5-HT₆ i3-7 (n=41 neurons). E) Relative levels of cAMP accumulation in IMCD3 cells transfected with empty vector (pCAGGS), WT 5-HT₆, Δ5-HT₆ No CTS, or Δ5-HT₆ i3-7; both in the presence and absence of WAY-208846 (n=3 independent biological replicates). * = P < 0.05, ** = P < 0.001, *** = P < 0.0001; 2-way ANOVA followed by post hoc Bonferroni test.