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. 2015 May 27;8(11):17593–17609. doi: 10.18632/oncotarget.4307

Figure 6. Pimozide suppresses the activity of STAT3 signaling and reverses cancer stem-like cell phenotypes induced by IL-6 addition in HCC cells.

Figure 6

A. MHCC-97L and Hep 3B cells were incubated with the different doses of pimozide for 48h before subjected to RT-PCR to analyse the expression of the STAT3 downstream genes c-Myc, Bcl-xL and Mcl-1. B. Western blot analysis of the expression of phosphorylated STAT3 (p-STAT3 Y705), STAT3 and c-Myc. C. HEK293T cells were transfected with STAT3 reporter and pRL-TK renilla luciferase reporter plasmids. After 24 hours of transfection, the cells were treated with different doses of pimozide for another 48h. Dual luciferase assay was performed to detect the relative luciferase activity. MHCC-97L cells were treated with 20 ng/ml IL-6, 10 μM pimozide or a combination as indicated for 12 h and subjected to western blot analysis (D), colony (E) and sphere (F) formation assays, and transwell migration assay (G) Relative representative figures are shown. The data are summarized from 3 independent experiments, *p < 0.05, **p < 0.01.