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. 2017 Feb 2;8(11):17873–17886. doi: 10.18632/oncotarget.15013

Figure 4. Targeting Hes1 impairs cell invasive abilities, modulates epithelial mesenchymal transition and inflammatory cytokines gene expression.

Figure 4

(A) Addition of EGF promoted a significant migration of pLKO.1 cells in cell invasion assay compared to stem medium, contrary to that occurs in shHes1-CSC. PDGFAA and PDGFBB promoted a significant migration of shHes1-CSC clones in comparison to stem medium, instead pLKO.1-CSC are not significantly influenced by PDGFAA and PDGFBB. Error bars represent the mean ± SD of two independent experiments performed in triplicate. *P values < 0.05, **P < 0.01**, ***P < 0.001 vs. control stem medium were considered statistically significant. (B) RT-qPCR analyses of key genes for angiogenesis, EMT, immunomodulation and cell invasion. mRNA expression for cell-substrate molecules interaction (CDH1, CDH5) were drastically downmodulated in shHes1-CSC vs pLKO.1 cells. Few EMT markers (TWIST1, FN1, VIM), together with immunomodulators (NOS2, IL6, IL1B, IL23, NF-κB2) and matrix metalloproteinases (MMP7, MMP9) were similarly downmodulated in shHes1-CSC in comparison to pLKO.1 cells.