Table 4. Primers and BNA/LNA oligonucleotides.
Oligo Name | Sequence | |
---|---|---|
A1 | BTK-FW | 5′-tgt aaa acg acg gcc agt CAG TTG TAT GGC GTC TGC AC-3′ |
A2 | BTK-REV | 5′-cag gaa aca gct atg acc TCC AGG TAT TCC ATG GCT TC-3′ |
A3 | BTK-BNA | 5′-G+GA+G+G+C+A+G+C+CAT+TG-[Phosphate]-3′ |
B1 | PLCγ2-Exon19-FW | 5′-tgt aaa acg acg gcc agt GCT CAC CTG GTC GTT TTC C-3′ |
B2 | PLCγ2-Exon19-REV | 5′-cag gaa aca gct atg acc CAA GCC CCT CTG TAG AGC AT-3′ |
B3 | PLCγ2-Exon19-LNA | 5′-+G+A+T+T+C+CC+C+G+G/3InvdT/-3′ |
C1 | PLCγ2-Exon20-FW | 5′-tgt aaa acg acg gcc agt AAA AAT TGT TTG GCC ACC AG-3′ |
C2 | PLCγ2-Exon20-REV | 5′-cag gaa aca gct atg acc TGG TGA ATA CTC AGA GGT TTG C-3′ |
C3 | PLCγ2-Exon20-BNA | 5′-G+G+AC+C+T+C+CG+C+CT-[Phosphate]-3′ |
D1 | PLCγ2-Exon24-FW | 5′-tgt aaa acg acg gcc agt AAA CGG TGT GCT TTG GAA AC-3′ |
D2 | PLCγ2-Exon24-REV | 5′-cag gaa aca gct atg acc AGA CAG GAC CCT GTG TCA GC-3′ |
D3 | PLCγ2-Exon24-LNA | 5′-+C+T+T+A+G+G+G+T+C+TC/3InvdT/-3′ |
Abbreviation: FW = forward, REV = reverse, +N = BNA/LNA bases.