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. 2017 Jan 31;8(11):17981–17994. doi: 10.18632/oncotarget.14912

Figure 1.

Figure 1

A. COX-2 expression in cells. B. PGE2 expression in cells; cells were exposed to 50 nM TPA for 24 h to induce COX-2 expression. C. Tumor volumes for COX-2 containing parental MDA-MB-231 (N=5) and COX-2 reduced Clone 13 (N=8) tumors; 2 × 106 cells were inoculated in 0.1 ml of 8.8 mg/ml Matrigel. D. Representative immunoblot showing COX-2 expression in MDA-MB-231 and Clone 13 tumors. GAPDH was used as loading control. E. Relative fold change in COX-2 mRNA levels in MDA-MB-231 (N=6) and Clone 13 (N=4) tumors. Values represent Mean ± SEM. *p ≤ 0.05; ***p ≤ 0.001 using ΔCt values.