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Representative 5 μm-thick H&E and corresponding α-SMA immunostained sections obtained from lungs of mice injected with A, B. MDA-MB-231 (N=5) and C, D. Clone 13 (N=3) cells. E. Spearman correlation between sum of metastatic nodule pixels (reflecting total nodule area) and sum of strongly positive pixels (reflecting number of CAFs) in lungs obtained from each mouse. A significant correlation was observed supporting the role of CAFs in the formation of metastasis.
