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. 2017 Feb 2;8(11):18095–18105. doi: 10.18632/oncotarget.14994

Figure 1. 5-Fluorouracil enhances TRAIL-induced apoptosis in lung adenocarcinoma cells.

Figure 1

A549 cells were pretreated with 5-fluorouracil at varying concentrations (0, 10, 20, and 40 μM) for 12 h followed by treatment with 200 ng/mL of TRAIL protein for an additional 2 h. A. Cell morphology photographed under light microscope in A549 Cells (×100); B. Cell viability determined with crystal violet assay in A549 Cells; C. Bar graph showing the average density of crystal violet dye in A549 Cells; D. Cell viability determined with trypan blue dye exclusion assays in A549 Cells. ** p < 0.01, *** p < 0.001: significant differences between control and each treatment group; 5-Flu: 5-Fluorouracil; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand.