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. 2017 Feb 9;8(11):18145–18153. doi: 10.18632/oncotarget.15210

Figure 2. The effect of CASC2 overexpression on cell viability and cell cycle.

Figure 2

A. The pcDNA-CASC2 significantly up-regulated the expression level of CASC2 in T24 and 5637 cells. B. MTS assay showed overexpression of CASC2 inhibited cell proliferation of T24 and 5637 cells. C. Colony formation assay measuring colony formation of CASC2 overexpression cells. Colony number was normalized to that obtained with cells transfected with empty vector, which was set to 100%. Overexpression of CASC2 significantly decreased the colony-forming ability of T24 and 5637 cells. D. Effect of CASC2 overexpression on the T24 and 5637 cell cycle. Cell cycle distribution was measured by propidium iodide staining followed by flow cytometry. The two cell had cell-cycle arrest at the G0-G1 phase compared with cells transfected with Empty vector. Each assay was performed in triplicate. Data are mean ± SD. * P<0.05, **P<0.01.