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. 2017 Feb 17;8(11):18312–18321. doi: 10.18632/oncotarget.15436

Figure 4. NPC-26 disrupts mitochondrial function, causing AMPK activation.

Figure 4

HCT-116 cells were treated NPC-26 (10 μM) for 3 hours, mitochondrial depolarization A. and cellar ROS content B. were tested. HCT-116 cells were pretreated for 30 min with NAC (500 μM), MnTBAP (10 μM), cyclosporin A (CsA, 0.5 μM), or sanglifehrin A (SfA, 2.5 μM), followed by NPC-26 (10 μM) treatment for indicated time period; Expression of listed proteins were shown C.; Cell survival D. and cell death E. were also tested. For each assay, n=5. Experiments in this figure were repeated three times, and similar results were obtained each time. AMPKα1/ACC phosphorylation (vs. total protein) was quantified (C). * p <0.05 vs. “C”. # p <0.05 vs. NPC-26 only.