Figure 3.

Proteins released in the culture supernatant of Mycoplasma hominis. A. Western blot analysis of the proteins from 150 μl of supernatant and cells from 15 μl of culture, immunostained with the membrane protein specific monoclonal antibodies BG11 (OppA), LF8 (P80) and CG4 (P60), BG2 (P50), and the cytoplasmic protein specific monoclonal antibody AH10 (P55). B. Western blot analysis of P60 and P80 of M. hominis lysate (Lys.) from 15 μl of culture and of two different fractions of the purified P60 (P60^SN) and P80 (P80^SN) from the supernatant of a cell culture using sepharose-coupled antibodies LF8 (P80) or CG4 (P60).