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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Cancer Res. 2017 Feb 16;77(8):1983–1996. doi: 10.1158/0008-5472.CAN-16-2246

Figure 6. EBP1 P48 inhibits FBXW7-mediated substrate-degradation.

Figure 6

A–C. HEK293T cells co-transfected with Flag-tagged EBP1 P48 or Flag-tagged EBP1 P42 and HA-tagged FBXW7α (A), ΔWD (B) or WD point mutant (C). The effect of EBP1 P48 on the subcellular distribution of FBXW7α was analyzed using Western blotting analysis. D. HCT116 FBXW7+/+ and HCT116 FBXW7−/− cells transfected with Flag-tagged EBP1 P48 respectively. The protein levels of indicated substrates of FBXW7 were analyzed by Western blotting. E. HEK293T cells were transfected with HA-tagged FBXW7α with or without Flag-tagged EBP1 P48. Immunoprecipitation with HA antibody followed by immunoblotting show that EBP1 P48 could decrease the binding of FBXW7 with its substrates, including cyclin E, c-Myc and Aurora A. All results are repeated at least three times. * P<0.05; ** P<0.01 based on Student t-test.