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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Pharmacol Res. 2017 Feb 22;119:289–302. doi: 10.1016/j.phrs.2017.02.016

Fig. 10.

Fig. 10

Effect of AdipoRon on PDGFR-β tyrosine phosphorylation and its association with p85 adaptor subunit of PI 3-kinase in VSMCs. A-B) Serum-deprived VSMCs were incubated with AdipoRon (25 μM) for 3 hr followed by stimulation with PDGF (30 ng/ml) for 6 min. VSMC lysates were then subjected to immunoblot analysis using primary antibodies specific for p-PDGFR-βTyr751 and PDGFR-β. GAPDH was used as internal control. C) Serum-deprived VSMCs were incubated with AdipoRon (50 μM) for 48 hr followed by stimulation with PDGF (30 ng/ml) for 2 min. The cell lysates were subjected to immunoprecipitation (IP) using PDGFR-β primary antibody and then probed with p85 primary antibody. The data shown in the bar graphs are the means ± SEM. *, # p < 0.05 compared with control (− PDGF and/or 0 μM AdipoRon) or PDGF (+ PDGF and 0 μM AdipoRon), respectively, using unpaired student t test (B) or two-way ANOVA followed by Bonferroni multiple comparisons test (C) [n = 3]. MC = Mock, HC = heavy chain.