Fig. 7.
Effects of AdipoRon on basal and PDGF-induced phosphorylation of Akt in VSMCs. Serum-deprived VSMCs were incubated with: A-B) AdipoRon (25 μM) for 3 hr or C-D) AdipoRon (50 μM) for 48 hr followed by stimulation with PDGF (30 ng/ml) for 6 min. VSMC lysates were subjected to immunoblot analysis using primary antibody specific for pAktSer473, pAktThr308 and Akt. β-actin was used as an internal control. The data shown in the bar graphs are the means ± SEM. * p < 0.05 using unpaired student t test (n = 3).