Fig. 9.

Time-dependent effects of AdipoRon on the phosphorylation of ERK, C-Raf, and MEK in VSMCs. Serum-deprived VSMCs were incubated with AdipoRon (25 μM) at increasing time intervals (6 min to 48 hr). VSMC lysates were subjected to immunoblot analysis using primary antibody specific for A) pERK and ERK or B) pC-Raf and C-Raf or pMEK and MEK. β-actin or GAPDH was used as internal controls. The data shown in the bar graphs are the means ± SEM. ^* p < 0.05 using repeated measures one-way ANOVA followed by Bonferroni multiple comparisons test (n = 3).