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. 2004 Nov 25;2:78. doi: 10.1186/1477-7827-2-78

Figure 1.

Figure 1

Localization of expression of mRNA encoding TGF-βs in ovine ovaries. Panels a and b contain corresponding light field and dark field views of a type 5 follicle from an adult ewe hybridized to TGF-β1 antisense RNA. Silver grains indicating hybridization of the TGF-β1 antisense RNA are observed concentrated in thecal (t) cells close to the basement membrane with no specific hybridization observed in either the granulosa cells (gc) or oocyte (o). The inset in panel b contains a dark field view of the same area of the tissue hybridized to the TGF-β1 sense RNA. Note the lack of specific concentration of silver grains over any cellular type. Panels c and d contain several type 5 (5) follicles and a type 4 (4) follicle in a 4 week old lamb hybridized to TGF-β2 antisense RNA. Note the lack of hybridization in the oocytes and granulosa cells of the type 4 and 5 follicles and the concentration of silver grains in thecal cells around the follicles as well as the stromal cells between the follicles and scattered cells of the surface epithelium (se). Observe the equal distribution of silver grains over the thecal cells. The inset in panel d contains a dark field view of the same area of the tissue hybridized to the TGF-β2 sense RNA. Note the lack of specific concentration of silver grains over any cellular type. Panels e and f contain corresponding light field and dark field views of an ovarian section obtained from an adult ewe hybridized to TGF-β3 antisense RNA. There is a lack of hybridization in the section including the granulosa and thecal cells of the types 4 (4) and 5 follicle (5) as well as the oocyte of the type 4 follicle, stroma tissue and the surface epithelium (SE). Panels g and h contain light field and dark field views of a blood vessel (v) from an adult ewe hybridized to TGF-β3 antisense RNA. Observe the specific hybridization in the wall of the vessel (v Inline graphic). Panel i contains a dark field view of the same area of the tissue hybridized to TGF-β3 sense RNA. Note the lack of specific concentration of silver grains over any cellular type. Scale bar equals approximately 100 μm for all panels.