Fig. 1. AQP4 overexpression in HEK293 cells using the AQP4-IRES2-EGFP vector. Western blot data confirming the successful transfection of AQP4. Lanes containing either untransfected HEK293 cells or HEK293 cells transfected with the IRES2-EGFP vector only do not show any bands. The lane containing lysate of HEK293 cells transfected with the M23-AQP4 gene shows a band at ~30 kDa. AQP4: aquaporin 4, EGFP: enhanced green fluorescent protein, HEK293: human embryonic kidney 293, IRES: internal ribosome entry site, N/C: untransfected cells, Vector: transfected IRES2-eGFP vector only.
