Figure 5.

Developmental and nutritional effects on candidate gene expression between C57BL/6J and 129S mice. C57BL/6J and 129S males were fed with a high fat diet (HF) or a standard diet (STD) from 3 or 8 weeks‐of‐age. Fat mass (A), fat‐free mass (B), adiposity (C), and mRNA levels (in 60 ng total RNA) of selected candidate genes including Sfrp5 (D–F), Mest (G–I), Scl25a1 (K–M), and Acly (O–Q) were measured by qRT‐PCR in three white fat depots (ING, EP, and RP) at 8, 19, and 31 weeks‐of‐age. Mesoderm‐specific transcript, SCL25A1, and ACLY protein (J, N, and R) in ING fats were analyzed by western blot at indicated time points. Data shown in graphs are the mean ± SD (error bars) with n = 7–13. Significant levels are indicated by *C57BL/6J group relative to 129S group in the same diet. Differences between groups were analyzed for statistical significance by Student's t‐test with significance levels indicated by *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.