Figure 7.

Developmental, genetic, and nutritional effects on histone acetylation at Sfrp5 and Acly in C57BL/6J and 129S mice. C57BL/6J and 129S males were fed with a high fat diet (HF) or a STD from 3 or 8 weeks‐of‐age. Glucose and insulin sensitivity of C57BL/6J (B–C) and 129S mice (D–E) were determined at 8, 19, and 31 weeks‐of‐age. Histone 2B, 3, and 4 acetylation at Sfrp5 and Acly genes was determined by ChIP assay in ING of adult C57BL/6J mice fed a STD diet (A). Adiposity, mRNA levels (in 60 ng total RNA) of Sfrp5 and Acly, acetylation of histone 3 and 4 associated with Sfrp5 and Acly in ING fats from C57BL/6J on STD (F–L) and HF (M–S), and 129S on STD (T–Z) and HF (AA–GG) mice were determined at the indicated time points. Data shown in graphs are the mean ± SD (error bars) with n = 3–4 (A, F‐GG) and 6–11 (B, C, D, and E). Differences between groups were analyzed for statistical significance by Student's t‐test (A, F‐GG) or two‐way ANOVA (B, C, D, and E) with multiple comparisons, significance levels indicated by *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001. STD, standard chow diet.