Fig. 1.

Study set-up showing the sample collection strategy (a) in which blood samples were collected before and just after 4 cycles of 5 min ischemia and 5 min reperfusion and study set-up for cell studies (b) adding either plasma or (R)/(S)-methyl-2-hydroxybutanoate (MHB) to buffer solutions during 40 min or 1 h stabilization (STAB), 5 h 30 min ischemia (ISC) and 2 h reperfusion (REP). Cellular damage was measured by propidium iodide/hoechst33342 (PI/HOE) staining