FIGURE 5.
Size exclusion chromatography of Kgp-NPD. Recombinant Kgp-NPD (1 mg/ml) was resolved on a SuperdexTM 75 increase 10/300 GL column equilibrated with 50 mm sodium acetate, 150 mm NaCl, pH 5.5 or 6.5, or with 50 mm Tris-HCl, 150 m NaCl, pH 8.0, with each buffer freshly supplemented with 2 mm dithiothreitol. The column was calibrated using conalbumin (75 kDa), ovoalbumin (43 kDa), carbonic anhydrase (29 kDa), and ribonuclease A (13.7 kDa), the respective elution volumes are indicated by vertical arrows above the chromatography profiles. For clarity, only the elution profiles at pH 5.5 and 8.0 are shown.