TABLE 2.
Primers employed in this study
Name | Sequence |
---|---|
Primers for Kgp mutagenesis | |
Kg_K129_Fs | ATCCCGAAAAGGTTCCCTT |
Kg_K129_Rs | AGGGTTGATGTGGCATGAG |
Kg_K129A_Rl | CATCACTGGCGCTCATAGAGGGTTGATGTGGCATGAG |
Kg_K129A_Fl | CTATGAGCGCCAGTGATGATCCCGAAAAGGTTCCCTT |
Kg_K129R_Rl | CATCACTACGGCTCATAGAGGGTTGATGTGGCATGAG |
Kg_K129R_Fl | CTATGAGCCGTAGTGATGATCCCGAAAAGGTTCCCTT |
Kg_K129E_Rl | CATCACTTTCGCTCATAGAGGGTTGATGTGGCATGAG |
Kg_K129E_Fl | CTATGAGCGAAAGTGATGATCCCGAAAAGGTTCCCTT |
Primers for Kgp-CepA plasmid construction | |
pUC_SphI_R | TGTGTGCATGCAAGCTTGGCGTAATCAT |
pUC_BamHI_F | TAGAAGGATCCCCGGGTACCGAGCT |
Kg_Up_F | AGCTTGCATGCACACACCCCGAT |
Kg_Up_R | ATGGAAGCTTAAGTCAGTCCAGCATGAGGAAG |
CepA_F | GACTTAAGCTTCCATAGACGATGCCACACTG |
CepA_R | GTTAAGATCTCAAGTCACCGATAGTGATAGTG |
Kg_Dw_F | ACTTGAGATCTTAACCTTGGTCTGCTCTAC |
Kg_Dw_R | CCGGGGATCCTTCTACCGTAACGTC |
Primers for qRT-PCR analysis | |
16S_F | AGGCAGCTTGCCATACTGCG |
16S_R | ACTGTTAGYAACTACCGATGT |
HrgpAForqPCR1 | ACGCTTCCCATTCTATCACG |
HrgpARevqPCR2 | CTCCCGGGAAGAATTTGTTT |
KgpForqPCR1 | AGCGCATGGATCTGAGACCGC |
KgpRevqPCR2 | CGGCATTAGCACCCACACTCCA |