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. 2017 Feb 14;292(14):5724–5735. doi: 10.1074/jbc.M117.776724

TABLE 2.

Primers employed in this study

Name Sequence
Primers for Kgp mutagenesis
    Kg_K129_Fs ATCCCGAAAAGGTTCCCTT
    Kg_K129_Rs AGGGTTGATGTGGCATGAG
    Kg_K129A_Rl CATCACTGGCGCTCATAGAGGGTTGATGTGGCATGAG
    Kg_K129A_Fl CTATGAGCGCCAGTGATGATCCCGAAAAGGTTCCCTT
    Kg_K129R_Rl CATCACTACGGCTCATAGAGGGTTGATGTGGCATGAG
    Kg_K129R_Fl CTATGAGCCGTAGTGATGATCCCGAAAAGGTTCCCTT
    Kg_K129E_Rl CATCACTTTCGCTCATAGAGGGTTGATGTGGCATGAG
    Kg_K129E_Fl CTATGAGCGAAAGTGATGATCCCGAAAAGGTTCCCTT

Primers for Kgp-CepA plasmid construction
    pUC_SphI_R TGTGTGCATGCAAGCTTGGCGTAATCAT
    pUC_BamHI_F TAGAAGGATCCCCGGGTACCGAGCT
    Kg_Up_F AGCTTGCATGCACACACCCCGAT
    Kg_Up_R ATGGAAGCTTAAGTCAGTCCAGCATGAGGAAG
    CepA_F GACTTAAGCTTCCATAGACGATGCCACACTG
    CepA_R GTTAAGATCTCAAGTCACCGATAGTGATAGTG
    Kg_Dw_F ACTTGAGATCTTAACCTTGGTCTGCTCTAC
    Kg_Dw_R CCGGGGATCCTTCTACCGTAACGTC

Primers for qRT-PCR analysis
    16S_F AGGCAGCTTGCCATACTGCG
    16S_R ACTGTTAGYAACTACCGATGT
    HrgpAForqPCR1 ACGCTTCCCATTCTATCACG
    HrgpARevqPCR2 CTCCCGGGAAGAATTTGTTT
    KgpForqPCR1 AGCGCATGGATCTGAGACCGC
    KgpRevqPCR2 CGGCATTAGCACCCACACTCCA
HHS Vulnerability Disclosure