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VWF D4-CK interacted with CUB1 and CUB2. A–C, binding between the VWF D4-CK domain fragment and the immobilized ADAMTS13 C-terminal domain fragments (CUB1, CUB2, and CUB1-2) was determined by SPR. Results are representative of three independent experiments. D, global analysis was used to determine KD and Bmax values. Results are presented as the mean ± S.D. (n = 3).
