Figure 9.

MDFIC alters glucocorticoid regulation of the inflammatory response in A549 cells. Nanostring analysis was performed on RNA isolated from A549 cells transfected with NTC siRNA or MDFIC siRNA (MDFIC-KD) and treated for 6 h with vehicle, 100 nm Dex, 10 ng/ml TNFα, or both 100 nm Dex and 10 ng/ml TNFα. A, RNA counts for the Ccl2 and nuclear factor κB subunit 1 (Nfkb1) genes. B, RNA counts for the Ccl20 and Csf1 genes. C, RNA counts for the C-C motif chemokine 19 (Ccl19) and prostaglandin I2 receptor (Ptgir) genes. Data shown are the raw RNA counts normalized to six housekeeping genes as described under “Experimental Procedures” and represent the mean ± S.E. from three independent experiments. A one-way ANOVA followed by Tukey's post hoc test was performed to determine significance. ***, p < 0.001 for TNFα versus con (vehicle). &, p < 0.05; &&&, p < 0.001 for Dex+TNFα versus TNFα. ##, p < 0.01. ###, p < 0.001 for MDFIC-KD Dex+TNFα versus NTC Dex+TNFα.