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ITC experiments for binding of ppGpp to ObgE_FL (a) and ObgE_340 (b), respectively. Experiments were performed at 25 °C by titrating ppGpp from a stock solution of 750 μm into a solution of 75 μm nucleotide-free ObgE. Equilibrium dissociation constants (KD) and binding stoichiometries (n) are given as the mean ± S.D. of 3 independent measurements (all isotherms are given in supplemental Fig. S9).
