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. 2017 Feb 24;292(14):5932–5942. doi: 10.1074/jbc.M116.761981

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 1. — A, co-translation and purification of untagged FERONIA with nanodiscs. a, elution from streptactin-Sepharose purification; b, flow-through from streptactin-Sepharose purification of reaction; c, soluble fraction from cell-free reaction; d, insoluble fraction from cell-free reaction. Top arrow, FERONIA band; bottom arrow, MSP band. B, FERONIA co-translated with nanodiscs exhibits high specific activity with MBP as a substrate; error bars, S.D., n = 3/bar, p = 0.00004. C, kinetic parameter determination for MBP. Curve fitting reveals the K_m and V_max for MBP to be 13.7 μm and 20.2 nmol/min/mg, respectively. Error bars, S.D.; n = 3/experimental data point.