Figure 5.

The grk-2 mutants can be rescued by inhibiting monoamine oxidase activity or by knocking out the monoamine oxidase amx-2. A, the monoamine oxidase inhibitor pargyline rescues the egg laying defect in both grk-2 mutants but not in egl-30(n686) (***, p < 0.001 compared with None). B, the knock-out of the monoamine oxidase ortholog amx-2 completely rescues the egg laying defect in grk-2(rt97) but not in grk-2(gk268) (*, p < 0.05; ***, p < 0.001 compared with grk-2(rt97)). C, Western blot shows AMX-2 is absent in the knock-out strain. Multiple transgenic lines show different expression levels of amx-2. D, the rescue of amx-2(ok1235) is dependent on the protein level (**, p < 0.01). All error bars indicate S.E. IB, immunoblotting.