Figure 2.

UbcH7-dependent transcriptional regulation. A, relative mRNA levels of indicated genes in A549 cells stably depleted of control shRNA or shUbcH7. Data were acquired as described under “Experimental Procedures” from two independent experiments in triplicate. Student's t test was used to determine the statistical significance (*, p < 0.001). B, A549 cells were infected with lentiviral particles targeting UbcH7 for 24, 48, 72, and 96 h. Relative mRNA levels of indicated genes were obtained using methods described under “Experimental Procedures.” Data were collected from two independent experiments in triplicate. *, p < 0.001. C, parallel samples from B were analyzed for protein expression. Protein quantitation was carried out as in Fig. 1A. D, A549 cells stably infected with control shRNA or shUbcH7 were treated with 500 nm CPT, 100 nm CTSLi, or vehicle for 8 and 12 h, respectively. The CTSL mRNA level was measured as described in A. Data represent mean and S.D. from two experiments in triplicate.