Figure 3.

UbcH7 regulated CTSL transcription. A, A549 control or UbcH7-depleted cells were treated with 1 μg/ml ActD for 0, 2, 4, and 8 h, RNAs were collected, and mRNA levels of Ube2l3 (the gene encoding UbcH7) and CTSL were measured by qPCR. Data represent the mean and S.D. from two independent experiments in duplicate. B, A549 control or UbcH7-depleted cells were cultured in regular media or without serum for 24 h, and gene expression levels were assessed as in A. Data represent the mean and S.D. from two independent experiments in duplicate.