FIGURE 1.

mga2Δ cells have reduced Sre1N accumulation in the presence and absence of oxygen. A and C, Western blots, probed with monoclonal anti-Sre1 IgG (5B4) and polyclonal anti-Dsc5 IgG (for loading) and imaged by LI-COR Biosciences Odyssey CLx, of lysates treated with alkaline phosphatase for 1 h from WT, mga2Δ, dsc2Δ, or sre1Δ cells grown for 0 or 4 h in the absence of oxygen (A) or 6 h in the statin CPN (200 μm) or vehicle (0.12% EtOH, 400 μm NaCl) (C). P and N denote precursor and cleaved N-terminal transcription factor forms, respectively. Asterisks denote nonspecific bands. B and D, quantification from A and C of three (A and B) or four (C and D) biological replicates normalized for loading to Dsc5 and then normalized to maximum signal (WT N terminus band after treatment; lane 2) for comparison between blots. Error bars are 1 S.D. (**, p < 0.01 for N terminus by two-tailed Student's t test). Quantities of the precursor and nuclear form are stacked to give an approximation of total signal per treatment. Average percent cleavage (clvg.) is calculated by dividing the quantity of N terminus by the sum of both N terminus and precursor.