TABLE 1.
Effect of PTX on agonist-induced BRET changes
ΔBRET in different conformational biosensors was measured in HEK 293 (top) or the ΔGαq/11/12/13 line (bottom) pretreated with 100 ng/ml PTX or vehicle for 16 h before FlAsH labeling and BRET assessment. Results of PTX effect on each sensor tested and their corresponding agonists are expressed as percentage of ΔBRET in relation to the value from vehicle-pretreated cells fixed at 100%. Values are averages ± S.E. of three independent experiments.
| Sensor/Agonist | Average effect ± S.E. | n |
|---|---|---|
| % of vehicle-treated cells | ||
| HEK 293 cells | ||
| ICL2P2/SI | 76.9 ± 1.2 | 3 |
| ICL3P3/Ang II | 76.1 ± 7.3 | 3 |
| C-tailP1/Ang II | 94.9 ± 18.1 | 3 |
| ΔGq,11,12,13 cells | ||
| ICL2P2/SI | 83.9 ± 3.4 | 3 |
| ICL3P3/Ang II | 59.2 ± 6.9 | 3 |
| C-tailP1/Ang II | 95.4 ± 9.6 | 3 |