TABLE 1.

Plasmids and E. coli strains used in this study

^a KAN is Kanamycin.

^b CAM is Chloramphenicol.

^c SM is Streptomycin.

^d SelC is native tRNA^Ser[Sec](UCA), while SelC2 made here is mutated tRNA^Ser[Sec](CUA).

^e See Ref. 33.

^f See Ref. 39.