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. 2017 Feb 15;292(13):5499–5506. doi: 10.1074/jbc.M116.769182

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Tafazzin changes the CL composition by combinations of forward and reverse transacylations. GST-tagged yeast tafazzin was incubated for 3 h with 0.72 mm PC, 0.08 mm MLCL (18:2)₃, and 0.12% Triton X-100 in a total volume of 0.1 ml. The transacylation products were analyzed by mass spectrometry. A, CL (18:2)₄ was the only transacylation product in the presence of PC 18:2/18:2, but multiple CL species were formed in the presence of PC 18:1/18:1. B, multiple CL and MLCL species were formed in the presence of PC 16:1/16:1. Data are mean values; error bars represent S.E. (n = 4). C, the scheme shows a sequence of transacylation reactions that convert PC 16:1/16:1 and MLCL (18:2)₃ into the transacylation products identified above.