TABLE 1.
Metal concentrations in 57Fe-enriched mouse hearts (and livers), and associated Mössbauer parameters
Metal concentrations are in μm, calculated by assuming a tissue density of 1.06 g/ml. Masses of hearts are in mg; uncertainties are ±1 mg. Values in parentheses refer to the number of hearts in the sample. If more than 1 heart was used, the mass refers to the average. Entry indicated with bold and italics is the collective mass of 4–7 hearts that had been combined. This mass, on a per-heart basis, is an outlier relative to the other samples. Sample designations C, H, and I refer to C57BL/6, HFE−/−, and IRP2−/− strains, respectively. The number that follows indicates the age of the mouse in weeks. Samples of the same age are distinguished with a and b. Samples from iron-deficient mice are distinguished by a subscript D. For ICP-MS analysis, each tissue was analyzed in triplicate; reported concentrations are averages; uncertainties are standard deviations. Cobalt and molybdenum concentrations were also determined but no age-dependent trends were apparent; average [Co] and [Mo] (n = 18) were 0.16 ± 0.11 μm and 0.05 ± 0.02 μm, respectively. Isomer shifts δ, quadrupole splittings ΔEQ, and line widths Γ (all in mm/s) used in simulations were: central doublet (0.45 ± 0.01, 1.15 ± 0.02, and 0.44 ± 0.07); low-temperature ferritin (−0.10 ± 0.05, 0.44 ± 0.07, and 0.77 ± 0.14) with Heff = 490 ± 6 kG; blood (0.91 ± 0.06, 2.30 ± 0.04, and 0.43 ± 0.11); high-temperature ferritin (0.44, 0.70, and 0.55). Mössbauer spectra were calibrated against α-iron foil at room temperature. For convenience, the sum of the percent relative intensities for the three major MB components were forced to 100%; however, 10–15% of the actual spectral intensity cannot be accounted for by these three components.
| Sample | Mass of heart | [57Fe] | [Fetot] | [Cu] | [Mn] | [Zn] | Central doublet | Ferritin sextet | Blood doublet |
|---|---|---|---|---|---|---|---|---|---|
| mg | %; μm | %; μm | %; μm | ||||||
| C00 (1 day) | 65 (1) | 240 ± 3 | 300 ± 8 | 10 ± 1 | 1.6 ± 0.2 | 31 ± 7 | 20; 60 | 20; 60 | 60; 180 |
| C01 | 100 | 350 ± 7 | 470 ± 10 | 33 ± 1 | 4.9 ± 0.1 | 43 ± 1 | 38; 180 | 17; 80 | 45; 210 |
| C02a | 180 (1) | 240 ± 9 | 290 ± 10 | 12 ± 0.4 | 2.3 ± 0.1 | 26 ± 1 | 53; 150 | 00; 00 | 47; 140 |
| C02b | 59 (2) | 170 ± 3 | 240 ± 9 | 19 ± 2 | 3.3 ± 0.1 | 26 ± 1 | 46; 110 | 13; 30 | 41; 100 |
| C03 | NDa | ND | ND | ND | ND | ND | 50; ND | 0; ND | 50; ND |
| C04a | 98 (4) | 600 ± 8 | 710 ± 9 | 29 ± 0.3 | 8.9 ± 0.2 | 46 ± 1 | 40; 280 | 10; 70 | 50; 360 |
| C04b | 61 (2) | 570 ± 10 | 610 ± 10 | 48 ± 1 | 11 ± 0.2 | 45 ± 1 | 44; 270 | 12; 70 | 44; 270 |
| C06 | 140 (1) | 620 ± 1 | 670 ± 10 | 42 ± 1 | 8.1 ± 0.1 | 43 ± 1 | 36; 240 | 17; 110 | 47; 320 |
| C12 | 150 (3) | 63 ± 1 | 610 ± 10 | 38 ± 1 | 5.8 ± 0.1 | 37 ± 1 | 31; 190 | 32; 190 | 37; 230 |
| C16 | ND | ND | ND | ND | ND | ND | 33; ND | 28; ND | 39; ND |
| C24 | 240 (2) | 950 ± 8 | 1050 ± 8 | 45 ± 1 | 7.7 ± 0.1 | 47 ± 1 | 30; 320 | 28; 290 | 42; 440 |
| C28 | 140 (2) | 740 ± 10 | 780 ± 10 | 36 ± 0.4 | 5.8 ± 0.1 | 34 ± 1 | 28; 220 | 36; 280 | 36; 280 |
| C52 | 250 (2) | 700 ± 10 | 1100 ± 20 | 39 ± 1 | 8.1 ± 0.1 | 48 ± 1 | 22; 240 | 48; 530 | 30; 330 |
| C60 | 130 (1) | 650 ± 30 | 960 ± 40 | 40 ± 10 | 3.4 ± 0.1 | 34 ± 1 | 16; 150 | 41; 390 | 43; 420 |
| C03D (pups) | 94 (4) | 260 ± 10 | 310 ± 20 | 40 ± 2 | 24 ± 1 | 56 ± 2 | 54; 170 | 00; 000 | 46; 140 |
| C28D (mom) | 390 (1) | 740 ± 5 | 850 ± 5 | 48 ± 0.3 | 8.8 ± 0.1 | 49 ± 1 | 35; 300 | 13; 110 | 52; 440 |
| H12 | 160 (3) | 58 ± 2 | 640 ± 20 | 58 ± 1 | 9.9 ± 0.2 | 60 ± 1 | 27; 170 | 37; 240 | 36; 230 |
| H12 (liver) | 1600 (1) | 1200 ± 200 | 5600 ± 700 | 29 ± 4 | 14 ± 2 | 90 ± 2 | 0.5; 30 | 90; 5040 | 9.5; 530 |
| C12 (liver) | 1300 (1) | 20 ± 90 | 960 ± 500 | 60 ± 30 | 14 ± 6 | 120 ± 70 | 18; 170 | 60; 580 | 22; 210 |
| I08 | 95 (2) | 520 ± 7 | 560 ± 7 | 35 ± 2 | 7.0 ± 0.1 | 32 ± 1 | 38; 210 | 10; 60 | 52; 290 |
| I104 | 200 (1) | 600 ± 20 | 800 ± 30 | 36 ± 1 | 7.7 ± 0.3 | 32 ± 1 | 30; 240 | 34; 270 | 36; 290 |
a ND, not determined.