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Functional characterization of 4E10 mutants: biological activity. A, PsV neutralization potency of the different mutants. The means of six measurements ± S.D. are represented in the plots. The experimental points were fitted to saturation curves. B, JRCSF PsV recognition as determined by dot blot analysis. Decreasing amounts of PsV (from left to right) were spotted onto nitrocellulose membranes and probed with 4E10 Fab WT and its mutants.
