TABLE 2.
Thermodynamic parameters of binding of Fab 4E10 mutants to the MPER(664–690) peptide and their neutralization potencies
The temperature was 298 K.
| Antibody (4E10) | KDa | ΔG°a | ΔH°a | −TΔS° | nb | HXB2- IC50c | JRCSF- IC50c |
|---|---|---|---|---|---|---|---|
| nm | kcal mol−1 | kcal mol−1 | kcal mol−1 | μg/ml | μg/ml | ||
| 4E10_WT | 4.6 ± 1.5 | −11.4 ± 0.2 | −15.1 ± 0.2 | 3.7 | 1.0 ± 0.1 | 0.21 ± 0.02 | 1.26 ± 0.09 |
| BS | 12.2 ± 2.5 | −10.8 ± 0.1 | −11.7 ± 0.1 | 0.9 | 1.3 ± 0.1 | 0.38 ± 0.03 | 1.59 ± 0.27 |
| R73E | 12.8 ± 3.5 | −10.8 ± 0.1 | −14.7 ± 0.2 | 3.9 | 1.1 ± 0.1 | 5.3 ± 0.4 | 13.2 ± 3.5 |
| K100eE | 91 ± 14 | −9.6 ± 0.2 | −10.4 ± 0.2 | 0.7 | 1.4 ± 0.1 | 3.3 ± 0.2 | 12.7 ± 4.0 |
aThe errors were calculated from the fitting procedure with ORIGIN 7.0.
bn refers to the molar ratio peptide/protein.
cThe values determined from the fitting curves in Fig. 4A.