TABLE 1.
LC-MS/MS confirmation of constituent proteins in the purified 2-oxoacid dehydrogenase complexes
ND, not detected; iBAQ, intensity-base absolute quantification.
| Banda | Unique peptidesb | Intensityc | iBAQd |
|---|---|---|---|
| Pyruvate dehydrogenase E3 | |||
| LpdG | 45 | 85.1% | 83.9% |
| Lpd3 | 9 | 0.030% | 0.035% |
| LpdV | 0 | ND | ND |
| α-Ketoglutarate dehydrogenase E2 | |||
| LpdG | 39 | 85.9% | 82.0% |
| Lpd3 | 0 | ND | ND |
| LpdV | 0 | ND | ND |
| Pyruvate dehydrogenase E2 | |||
| AceE | 46 | 80.4% | 81.6% |
a Band labels correspond to the gel shown in Fig. 5A. Each sub-row shows information about proteins of interest for a given gel band.
b Number of unique peptides observed for the indicated protein within the indicated band is shown.
c Percent peptide abundance was based on the raw signal intensity of identified peptides.
d Percent peptide abundance using the iBAQ method (26) is shown.