Figure 2.

RNA polymerase II RPB1 phosphorylation in the striatum of mice trained in a fast lever-pressing task. A, Immunoblot analysis of the total RPB1 CTD repeat YS₂PTS₅PS₇, with indication of its hyperphosphorylated (II₀) and hypophosphorylated (II_A) forms, Ser5P⁺-enriched RPB1 CTD and Ser2P⁺-enriched RPB1 CTD. B, Actin quantification across both phospho-isoforms. C, RNA polymerase II poising index (calculated as the quotient between the Ser5-P and Ser2-P RPB1 CTD phospho-isoforms) in the striatum of mice trained in the fast lever pressing. For both control and trained groups, n = 7; data as mean ± SEM; ***p < 0.005.