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. Author manuscript; available in PMC: 2017 Apr 17.
Published in final edited form as: Cell Rep. 2017 Apr 4;19(1):150–161. doi: 10.1016/j.celrep.2017.03.022

Figure 2. Glo qRRMs bind to a single-stranded G-tract sequence.

Figure 2

Binding of purified recombinant Glo qRRM1, qRRM2, qRRM3, and qRRM1,2 proteins to radiolabeled G-tract (5′-AGGGA) RNA. Representative EMSA gels are shown, with data plotted below. The highest protein concentration (right-most lanes) was 3.1 μM and 2-fold serial dilutions were assayed. No protein was added to the reactions in the lanes marked ‘-’. The positions of unbound RNA (asterisk) and Glo qRRM:RNA complexes (arrowhead) are indicated. Three technical replicates of EMSAs were performed, and apparent Kd values shown are mean ± SEM.