FIGURE 5.

SHIP-1 modulation reduces CXCL11-mediated migratory responses in activated T lymphocytes. (A) Previously SEB-activated T cells were treated with AQX1 (1–30 μM) for 30 min. Basal and chemotactic migration to CXCL11 (10 nM) was assessed using the Neuro Probe chemotaxis assay as described in Materials and Methods. Data are expressed as percentage inhibition of migration and are means ± SEM of three independent experiments. (B–D) Additionally, the migration of previously activated T cells treated with AQX1 (30 μM) with or without CXCL11 (100 nM) gradient was also assessed using ibidi two-dimensional chemotaxis μ-slides and video microscopy as described in Materials and Methods. Data represent three separate experiments with 20 cells recorded under each condition in each experiment, where (B) accumulated distance (micrometers) and (C) velocity (units/second) are the means ± SEM from three independent experiments. (D) Individual tracks of cell tracks from a single representative experiment. *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA with a Dunnett posttest).