FIGURE 6.

SHIP-1 modulation inhibits polarization and alters the phosphorylation state of ERM proteins in T lymphocytes. (A) Previously activated T lymphocytes were incubated either with vehicle control (labeled C) or AQX1 (3–30 μM) for 30 min and then stimulated with CXCL11 (10 nM) as indicated for 5 min. Levels of phosphorylated ERM, phosphorylated Akt, and total ERK were assessed using immunoblotting. The upper panel is a representative Western blot from a single experiment. The right panel shows the mean ± SEM of phosphorylated ERM relative to total ERK, and the left panel shows the mean ± SEM of phosphorylated Akt relative to total ERK from three independent experiments. *p < 0.05, **p < 0.01 (one-way ANOVA with a Dunnett posttest). (B) Previously activated T cells were treated with AQX1 (30 μM) and allowed to equilibrate upon poly-l-lysine (0.1 mg/ml)–coated ibidi μ-slides. Cells were stimulated with CXCL11 (100 nM) and lamellipodia extension observed using the Zeiss LSM 510 META microscope.